Welcome to Protein Chemical Biology and Bioorthogonal Chemistry Lab!


Research interests:

Development and application of bioorthogonal chemical reactions on living systems, to provide innovative tools for life science research and develop innovative technologies for diagnostics and therapeutics through the chemical knowledge and tools.
1.Bioorthogonal chemistry: development and application of bioorthogonal chemistry and chemical tools for life sciences.
2.Protein engineering and omics: protein engineering and multi-omics technology.
3.Immune chemical biology: interactions between biomacromolecules as well as cells in the regulations and interventions of immune systems.
(See research page for more details!)

Selected References:

1, Ngai, W. S. C.; Yang, S.; Zeng, X.; Liu, Y.; Lin, F.; Wang, X.; Zhang, H.; Fan, X.; Chen, P. R., Bioorthogonally Activatable Base Editing for On-Demand Pyroptosis. J. Am. Chem. Soc. 2022, DOI: 10.1021/jacs.1c12924.

2, Liu, H.; Luo, H.; Xue, Q.; Qin, S.; Qiu, S.; Liu, S.; Lin, J.; Li, J. P.; Chen, P. R., Antigen-Specific T Cell Detection via Photocatalytic Proximity Cell Labeling (PhoXCELL). J. Am. Chem. Soc. 2021, DOI: 10.1021/jacs.2c00159.

3, Liu, S.; Lin, C.; Xu, Y.; Luo, H.; Peng, L.; Zeng, X.; Zheng, H.; Chen, P. R.; Zou, P., A far-red hybrid voltage indicator enabled by bioorthogonal engineering of rhodopsin on live neurons. Nat. Chem. 2021, 13 (5),472-479.

4, Huang, Z.; Liu, Z.; Xie, X.; Zeng, R.; Chen, Z.; Kong, L.; Fan, X.; Chen, P. R., Bioorthogonal Photocatalytic Decaging-Enabled Mitochondrial Proteomics. J. Am. Chem. Soc. 2021, 143 (44),18714-18720.

5, Zhang, H.; Han, Y.; Yang, Y.; Lin, F.; Li, K.; Kong, L.; Liu, H.; Dang, Y.; Lin, J.; Chen, P. R., Covalently Engineered Nanobody Chimeras for Targeted Membrane Protein Degradation. J. Am. Chem. Soc. 2021, 143 (40),16377-16382.

6, Wang, J.; Wang, X.; Fan, X.; Chen, P. R., Unleashing the Power of Bond Cleavage Chemistry in Living Systems. ACS Cent. Sci. 2021, 7 (6), 929-943.

7, Liu, Y.; Zeng, R.; Wang, R.; Weng, Y.; Wang, R.; Zou, P.; Chen, P. R. Spatiotemporally resolved subcellular phosphoproteomics. Proc. Natl. Acad. Sci. USA. 2021, 118(25), e2025299118.

8, Song H, Wang Y, Wang R, Zhang X, Liu Y, Jia G, Chen P. SFPQ Is an FTO-Binding Protein that Facilitates the Demethylation Substrate Preference. Cell Chem. Biol. 2020, 27, 283-91.

9, Wang, J.; Liu, Y.; Liu, Y.; Zheng, S.; Wang, X.; Zhao, J.; Yang, F.; Zhang, G.; Wang, C.; Chen, P. R. Time-resolved protein activation by proximal decaging in living systems. Nature 2019, 569 (7757), 509-513.

10, Zhang S, He D, Yang Y, Lin S, Zhang M, Dai S, Chen P. Comparative proteomics reveal distinct chaperone-client interactions in supporting bacterial acid resistance. Proc. Natl. Acad. Sci. USA. 2016, 113, 10872-7.