赵美萍

学术简历

• 2010 –至今   教授，北京大学化学与分子工程学院
• 2002 –2010   副教授，北京大学化学与分子工程学院
• 1998.7-1998.11   访问学者 荷兰能源研究机构 环境分析部
• 1997.9–1998.6   访问学者 荷兰阿姆斯特丹大学 环境与毒理化学系
• 2002   理学博士 北京大学 化学与分子工程学院
• 1993   理学硕士 北京大学 环境科学中心
• 1990   理学学士 北京大学 技术物理系

科研领域及兴趣

本课题组致力于探索和构建新颖的分子识别体系，发展实时、在线的荧光分析方法，为生命科学研究、临床检验、环境监测和食品安全监控提供高效的分析测量手段。目前的主要研究方向：

1. 核酸酶与核酸相互作用的分子机理研究；
2. 活细胞的不同亚细胞区室内核酸修复蛋白的原位荧光成像和结构解析；
3. 基因损伤、基因突变、多重微生物基因序列的超高灵敏检测；
4. 多聚蛋白分子印迹识别材料的制备方法和应用。

代表性论文

1. XJ Cao, JH Zheng, RL Zhang, Y Sun, MP Zhao*, Live-cell imaging of human apurinic/apyrimidinic endonuclease 1 in the nucleus and nucleolus using a chaperone@DNA probe, Nucleic Acids Research, 2024, 52(8), e41.
2. W Chen,HQ Xu, SB Dai, JY Wang, ZY Yang, YW Jin, MB Zou, XJ Xiao*, TB Wu*, W Yan, B Zhang, ZM Lin, MP Zhao*, Detection of low-frequency mutations in clinical samples by increasing mutation abundance via the excision of wild-type sequences, Nature Biomedical Engineering, 2023, 7, 1602. (https://rdcu.be/dhUlL).
3. ZY Yang, W Chen, JY Wang, MH Shi, RL Zhang, SB Dai, TB Wu*, MP Zhao*, Programmable One-Pot Enzymatic Reaction for Direct Fluorescence Detection of Ultralow-Abundance Mutations in the DNA Duplex, Analytical Chemistry, 2021, 93, 7086.
4. JQ Zhai, MH Zhao, XJ Cao, MY Li*, MP Zhao*, Metal-ion-responsive bionanocomposite for selective and reversible enzyme inhibition, Journal of the American Chemical Society, 2018, 140, 16925.
5. TB Wu, YF Yang, W Chen, JY Wang, ZY Yang, SL Wang, XJ Xiao, MY Li, MP Zhao*, Noncanonical substrate preference of lambda exonuclease for 5'-nonphosphate-ended dsDNA and a mismatch-induced acceleration effect on the enzymatic reaction, Nucleic Acids Research, 2018, 46, 3119.
6. TB Wu, W Chen, ZY Yang, HC Tan, JY Wang, XJ Xiao, MY Li, MP Zhao*, DNA terminal structure-mediated enzymatic reaction for ultra-sensitive discrimination of single nucleotide variations in circulating cell-free DNA, Nucleic Acids Research, 2018, 46(4), e24.
7. XJ Xiao, TB Wu, L Xu, W Chen, MP Zhao*, A branch-migration based fluorescent probe for straightforward, sensitive and specific discrimination of DNA mutations, Nucleic Acids Research, 2017, 45 (10): e90.
8. JQ Zhai, YB Liu, S Huang, SM Fang, MP Zhao*, A specific DNA-nanoprobe for optically tracking the activities of human apurinic/apyrimidinic endonuclease 1 in living cells, Nucleic Acids Research, 2017, 45(6), e45.
9. XJ Xiao, TB Wu, FD Gu, MP Zhao*, Generation of artificial sequence-specific nucleases via preassembled inert-template, Chemical Science, 2016, 7, 2051.
10. SM Fang, L Chen, MP Zhao*, Unimolecular chemically modified DNA fluorescent probe for one-step quantitative measurement of the activity of human apurinic/ apyrimidinic endonuclease 1 in biological samples, Analytical Chemistry, 2015, 87, 11952.
11. ZY Chen, L Xu, Y Liang, MP Zhao*, pH-Sensitive water-soluble nanospheric imprinted hydrogels prepared as horseradish peroxidase mimetic enzyme, Advanced Materials, 2010, 22, 1488.
12. C Song, MP Zhao*, Real-time monitoring of the activity and kinetics of T4 polynucleotide kinase by a singly labeled DNA-hairpin smart probe coupled with  exonuclease cleavage, Analytical Chemistry, 2009, 81, 1383.