箴言
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在科学上没有平坦的大道,只有那些不畏艰险沿着陡峭山路攀登的人,才有希望达到光辉的顶点。
----马克思
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合作研究
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请有兴趣的研究组联系我们。欢迎任何形式的合作，尤其是在自组装、水凝胶以及生物医药等方向的合作。
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请有兴趣的研究组联系我们。欢迎任何形式的合作，尤其是在自组装、水凝胶以及生物医药等方向的合作。
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研究成果
85. Supercharging SpyCatcher toward an Intrinsically Disordered Protein with Stimuli-Responsive Chemical Reactivity. Chem. Commun. 2017, 53, 8830-8833 (Front Cover)
发布时间:2017-10-18
Cao, Y.; Liu, D.; Zhang, W.-B.* Supercharging SpyCatcher toward an Intrinsically Disordered Protein with Stimuli-Responsive Chemical Reactivity. Chem. Commun. 2017, 53, 8830-8833 (Front Cover). [Link]
Abstract
We report a supercharged, intrinsically disordered protein, SpyCatcher(−), possessing stimuli-responsive reactivity toward SpyTag with tunable yields ranging from 4% to 98% depending on pH, temperature, ionic strength, etc. The CD and NMR studies reveal that the reaction occurs through a folded intermediate formed probably via a different mechanism from that of SpyCatcher.