Single bio-molecule detection

Life is fascinating. Each cell is a well-organized factory. In such a factory, some duties are carried out by only a few of molecules. Nano-science and technology are fascinating. A few, or even one single molecule may form a device, which functions just as well as a huge machine. People explain the world in terms of atoms and molecules. But, only very recently one can detect the single molecule.

                By using confocal fluorescence spectroscope and other techniques, we are able to track the motion of single molecule. Currently, we are working on molecular motion and interaction in living cell by single molecule detection. Study of these processes allows us not only to reveal the secret of life, but also to find novel biotechnologies.

Bio-molecule recognition

         References
12. Wenjuan Wang, Chunlai Chen, Minxie Qian, Xin Sheng Zhao, Anal. Biochem. 373, 213-219 (2008). "Aptamer biosensor for protein detection using gold nanoparticles".
11. Chunlai Chen, Wenjuan Wang, Zhang Wang, Fang Wei and Xin Sheng Zhao, Nucleic Acids Res. 35, 2875–2884 (2007). "Influence of secondary structure on kinetics and reaction mechanism of DNA hybridization".
10. Z hang-Yi Liang, Ning Xu, Ying-Hua Guan, Ming Xu, Qi-Hua He, Qi-De Han, You-Yi Zhang, Xin Sheng Zhao, Biochem. Biophys. Res. Commun. 353. 231–237 (2007) . "The transport of a1A-adrenergic receptor with 33-nm step size in live cells".
9. Yinghua Guan, Zheng Wang, Aoneng Cao, Luhua Lai, and Xin Sheng Zhao, J. Am. Chem. Soc. 128, 7203-7208 (2006). "Subunit Exchange of MjHsp16.5 Studied by Single-Molecule Imaging and Fluorescence Resonance Energy Transfer".
8. Wei Liao, Fang Wei, Dan Liu, Min Xie Qian, Gu Yuan, Xin Sheng Zhao, Sensors and Actuators B 114, 445-450 (2006). "FTIR-ATR detection of proteins and small molecules through DNA conjugation".
7．Fang Wei, Chunlai Chen, Lin Zhai, Ning Zhang, and Xin Sheng Zhao, J. Am. Chem. Soc. 127, 5306 (2005). "Recognition of Single Nucleotide Polymorphisms using Scanning Potential Hairpin Denaturation". and supporting information.
6．Jian Hua Ouyang and Xin Sheng Zhao, Proceedings of SPIE 5593, 247 (2004). "Patterning Silicon Surfaces with Covalently attached DNA at Sub-Micrometer Resolution".
5． Wei Liao, Fang Wei, Min Xie Qian, Xin Sheng Zhao, Sensors and Actuators B 101, 361 (2004). "Characterization of protein immobilization on alkyl monolayer modified silicon(111) surface".
4． Fang Wei, Bin Sun, Wei Liao, Jianhua Ouyang, Xin Sheng Zhao, Biosensors & Bioelectronics 18, 1149 (2003). "Achieving Differentiation of Single-base Mutations through Hairpin Oligonucleotide and Electric Potential Control".
3． Lizhen Ying, Xu Huang, Bo Huang, Jia Xie, Jindong Zhao, Xin Sheng Zhao, Photochem. Photobio. 76, 310 (2002). "Fluorescence Emission and Adsorption Spectra of Single Anabaena sp. Strain PCC7120 Cells".
2． Liu Tingting, Luo Guobin, Ying Liming, and Zhao Xinsheng, Acta Phys. Chim. Sin. 16, 393 (2000). "Single-Molecule Detection Studies of Diffusion of Phodamine-labeled Phospholipid".
1． Todd Strother, Wei Cai, Xinsheng Zhao, Robert J. Hamers, and Lloyd M. Smith, J. Am. Chem. Soc. 122, 1205 (2000). "Synthesis and Characterization of DNA-Modified Silicon (111) Surfaces".